The ideal red blood cells shelf life is 21 days when stored in ACD solution. Red blood cells in ACD solution utilize glucose and their viability impairs when re-infused.

 

The ACD Solution A contains Citric acid (anhydrous) 7.3 g/L, Sodium citrate (dihydrate) 22.0 g/L and Dextrose (monohydrate) 24.5 g/L . Citrate-based anticoagulants prevent the coagulation of blood by virtue of the citrate ion's ability to chelate ionized calcium present in the blood to form a non-ionized calcium-citrate complex.

 

Our Acid Citrate Dextrose (ACD) Solution A is supplied as a high quality, 0.22µm filter sterilized ready to use solution, packaged in sterile bottles for use as an anticoagulant in research studies only.

 

Applications

Acid-Citrate-Dextrose solution (ACD) is used for dilution of blood for haematological studies.

Used as an anticoagulant during blood collection by cardiac puncture from mice and human bone marrow isolation.

 

ACD is available in two formulations. 

Both solutions are comprised of trisodium citrate, citric acid and dextrose. 

The formulations are as follows:

	ACD Solution A	ACD Solution B
Trisodium Citrate	22.0g/L	13.2g/L
Citric Acid	8.0g/L	4.8g/L
Dextrose	24.5g/L	14.7g/L

The International Council for Standardization in Haematology and NCCLS have recommended K2EDTA as the anticoagulant of choice for blood cell counting and sizing for the following reasons1,2:

• K3EDTA results in greater RBC shrinkage with increasing EDTA concentrations

(11% shrinkage with 7.5 mg/ml blood).

• K3EDTA produces a larger increase in cell volume on standing (1.6% increase after 4 hours).

• K3EDTA leads to lower MCV values (typically a -0.1 to -1.3% difference is observed compared with K2EDTA).

• K3EDTA is a liquid additive, and therefore, will result in the dilution of the specimen. All directly measured values (Hgb, RBC, WBC, and platelet counts) have been reported to be 1-2% lower than results obtained with K2EDTA2,3.

• With some instrument systems, K3EDTA gives lower WBC counts when used at high concentrations. Brunson, et al., reported that plastic tubes containing K2EDTA gave complete blood count and differential results in excellent agreement with glass tubes containing K3EDTA, though they confirmed the earlier results of 1-2% higher WBC, RBC, hemoglobin, and platelet count results with the former tube, owing to dilution observed with K3EDTA4.

• Our internal studies showed no clinically significant differences when comparing K3EDTA glass tubes to K2EDTA plastic tubes.